Journal: Pharmaceutics

Article Title: Lipid Systems for the Delivery of Amphotericin B in Antifungal Therapy

doi: 10.3390/pharmaceutics12010029

Figure Lengend Snippet: Lipid-based systems for delivery of amphotericin B currently under development.

Article Snippet: Acetylated Sterculia striata polysaccharide (ASSP) NCs(DS 1.68 and DS 1.35) , N/A , NC1.68: 274.1 ± 8.6 (0.181); NC1.35: 277.3 ± 9.5 (0.149) , NC1.68: 99.2 ± 1.3; NC1.35: 80.5 ± 1.6 , ASSP (1.0 mg/mL), Miglyol812 ® , AmB (0.25 mg/mL). Spontaneous emulsification. , NC1.68 (ASSP concentration, 0.5 mg/mL) and NC1.35 (ASSP concentration, 1.0 mg/mL) loaded AmB in a monomeric form. AmB-NC1.68: MIC 0.25 μg/mL against 5 strains of C. albicans (ATCC 90028, LABMIC 0104, LABMIC 0105, LABMIC 0106). In vitro controlled release of AmB: 3.6 ± 0.5% after 1 h; 49.1 ± 0.4% after 72 h; complete release after 212 h. NC1.68 showed potential to be employed as AmB DDS. , [ ] .

Techniques: Formulation, Conjugation Assay, Permeability, In Vitro, Activity Assay, Produced, MTT Assay, In Vivo, Concentration Assay, Spectroscopy, Shear, Liposomes, Modification, Dispersion, Injection, Incubation, Saline, Sonication, Infection, Solubility, Encapsulation, Homogenization, Titration, Ex Vivo, Viscosity, Inhibition, Diffusion-based Assay, Emulsion, Clinical Proteomics, Fluorescence, Imaging, Adjuvant, Expressing, RNA Expression, Solvent, Control, Histopathology, Polymer, Evaporation, Emulsification, Eye Drops

Journal: PLoS ONE

Article Title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase

doi: 10.1371/journal.pone.0201369

Figure Lengend Snippet: (A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by CellTiter-Glo ® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E ( αT ), its metabolites ( αTQ , αTHQ ), or the synthetic carbochroman ( αTCC ) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPY TM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.

Article Snippet: Mouse striatal Q7 cells (ST Hdh Q7/Q7 ; immortalized by SV large T antigen) were obtained from Coriell.

Techniques: ATP Assay, Activity Assay, Titration, Concentration Assay, Fluorescence, Time-lapse Microscopy

Journal: PLoS ONE

Article Title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase

doi: 10.1371/journal.pone.0201369

Figure Lengend Snippet: (A) To assess the in vivo conversion of αT to its quinone metabolite, stable deuterium-labelled α-tocopherol (d 4 - αT ) was dosed orally to Sprague-Dawley male rats. Four hours after the final dose, plasma and tissues were collected for bioanalytical quantification of d 4 - αT and d 4 - αTQ by LC-MS/MS. While <1% of deuterium-labeled αT was detected as the quinone in the plasma fraction, varying levels of quinone conversion were observed in the tissues assessed, ranging from ~2% (liver) to ~50% (small intestine). (B) Vitamin E ( αT ) and its metabolites, vitamin E quinone ( αTQ ) and vitamin E hydroquinone ( αTHQ ), were detected simultaneously in Q7 striatal cells under basal growth conditions or supplementation with αT or αTQ (10 μM, 24 h). Results displayed are mean ± SD, n = 6; results from 1 experiment representative of 3 similar experiments. (C) Stacked bar graphs showing the mean proportions of αT , αTQ , and αTHQ quantified simultaneously under basal, αT - or αTQ -supplemented conditions using the succinate capping methodology described in Methods.

Article Snippet: Mouse striatal Q7 cells (ST Hdh Q7/Q7 ; immortalized by SV large T antigen) were obtained from Coriell.

Techniques: In Vivo, Liquid Chromatography with Mass Spectroscopy, Labeling

Journal: PLoS ONE

Article Title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase

doi: 10.1371/journal.pone.0201369

Figure Lengend Snippet: (A) RSL3-Q7 treated cells have elevated cellular levels of a lyso-PE-palmitoyl species (*p<0.05, Unpaired t-test with Welch’s correction). (B, C) RSL3-treated Q7 cells have elevated cellular and conditioned media levels of 15-HETE, an effect prevented by αTQ (5 μM) co-treatment (*p<0.05, **p<0.01, ***p<0.001, Shapiro-Welk normality test followed by ANOVA with Sidak’s multiple comparisons test or Kruskal-Wallis test with Dunn’s multiple comparisons test, respectively). (D, E) 12-HETE levels were not altered by RSL3 or αTQ treatment (ns, p>0.05, Shapiro-Welk normality test followed by Kruskal-Wallis test with multiple comparisons test. (F, G) Conditioned medium of RSL3-treated Q7 cells has elevated AA, but cellular levels are not significantly changed (***p<0.001, Shapiro-Welk normality test followed by ANOVA with Sidak’s multiple comparisons test).

Article Snippet: Mouse striatal Q7 cells (ST Hdh Q7/Q7 ; immortalized by SV large T antigen) were obtained from Coriell.

Techniques: